Janelia Fluor 549
Janelia Fluor® 549 (Ex-Max 549 nm/Em-Max 571 nm)
Janelia Fluor® 549 was developed at the Janelia Campus of the Howard Hughes Medical Institute but is commercialized by other vendors. The Janelia Fluor®s family is unique in that the fluorophores are cell-permeable and are available in photoactivatable forms. These fluorophores were developed for super-resolution microscopy (STED, PALM and STORM) and live-cell microscopy in the HaloTag and SNAP-tag versions. Janelia Fluor® 549 has an excitation peak at 549 nm and an emission peak at 571 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 549 nm
- Emission maximum
- 571 nm
- Optimal laser line
- 532
- Optimal filter
- 586/25
- Relative brightness
- 2 of 5
- Extinction coefficient
- 101000
- Quantum yield
- 0.88
- Commercial antibodies
- 22.4k from 1 supplier
Janelia Fluor 549 has an excitation maximum at 549 nm and an emission maximum at 571 nm, a Stokes shift of 22 nm. Its extinction coefficient is 101000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is Janelia Fluor 549?
- Janelia Fluor® 549 was developed at the Janelia Campus of the Howard Hughes Medical Institute but is commercialized by other vendors. The Janelia Fluor®s family is unique in that the fluorophores are cell-permeable and are available in photoactivatable forms. These fluorophores were developed for super-resolution microscopy (STED, PALM and STORM) and live-cell microscopy in the HaloTag and SNAP-tag versions. Janelia Fluor® 549 has an excitation peak at 549 nm and an emission peak at 571 nm.
- What laser excites Janelia Fluor 549?
- Janelia Fluor 549 is optimally excited by the 532 laser line. Its excitation maximum is 549 nm.
- What filter should I use to detect Janelia Fluor 549?
- A 586/25 filter captures the peak of the Janelia Fluor 549 emission near 571 nm with minimal spillover.
- How bright is Janelia Fluor 549?
- Janelia Fluor 549 has a relative brightness of 2 out of 5 (quantum yield 0.88).
- How many antibodies are available conjugated to Janelia Fluor 549?
- FluoroFinder tracks 22383 commercially available Janelia Fluor 549 conjugates, searchable by target, clone, host and application.