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PromoFluor 488P

PromoFluor 488P (Ex-Max 491 nm/Em-Max 517 nm)

PromoFluor 488P is a fluorophore with an excitation peak at 490 nm and an emission peak at 516 nm. It is equivalent to Alexa Fluor™ 488 and DyLight™ 488 in size, brightness and application. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.

Excitation & emission spectra

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Key facts

Excitation maximum
491 nm
Emission maximum
517 nm
Optimal laser line
488
Optimal filter
530/30
Relative brightness
2 of 5
Quantum yield
0.896
Commercial antibodies
19 from 2 suppliers

PromoFluor 488P has an excitation maximum at 491 nm and an emission maximum at 517 nm, a Stokes shift of 26 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is PromoFluor 488P?
PromoFluor 488P is a fluorophore with an excitation peak at 490 nm and an emission peak at 516 nm. It is equivalent to Alexa Fluor™ 488 and DyLight™ 488 in size, brightness and application. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.
What laser excites PromoFluor 488P?
PromoFluor 488P is optimally excited by the 488 laser line. Its excitation maximum is 491 nm.
What filter should I use to detect PromoFluor 488P?
A 530/30 filter captures the peak of the PromoFluor 488P emission near 517 nm with minimal spillover.
How bright is PromoFluor 488P?
PromoFluor 488P has a relative brightness of 2 out of 5 (quantum yield 0.896).
How many antibodies are available conjugated to PromoFluor 488P?
FluoroFinder tracks 19 commercially available PromoFluor 488P conjugates, searchable by target, clone, host and application.