PromoFluor 633P
PromoFluor 633P (Ex-Max 637 nm/Em-Max 657 nm)
PromoFluor 633P is a fluorophore with an excitation peak at 635 nm and an emission peak at 658 nm. It is equivalent to Alexa Fluor™ 633 and Cy5 in size, brightness and application. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 637 nm
- Emission maximum
- 657 nm
- Optimal laser line
- 638
- Optimal filter
- 670/30
- Relative brightness
- 3 of 5
- Quantum yield
- 0.127
PromoFluor 633P has an excitation maximum at 637 nm and an emission maximum at 657 nm, a Stokes shift of 20 nm. It is optimally excited by the 638 laser line.
Frequently asked questions
- What is PromoFluor 633P?
- PromoFluor 633P is a fluorophore with an excitation peak at 635 nm and an emission peak at 658 nm. It is equivalent to Alexa Fluor™ 633 and Cy5 in size, brightness and application. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.
- What laser excites PromoFluor 633P?
- PromoFluor 633P is optimally excited by the 638 laser line. Its excitation maximum is 637 nm.
- What filter should I use to detect PromoFluor 633P?
- A 670/30 filter captures the peak of the PromoFluor 633P emission near 657 nm with minimal spillover.
- How bright is PromoFluor 633P?
- PromoFluor 633P has a relative brightness of 3 out of 5 (quantum yield 0.127).