PromoFluor 680
PromoFluor 680 (Ex-Max 690 nm/Em-Max 710 nm)
PromoFluor 680 is a fluorophore with an excitation peak at 690 nm and an emission peak at 709 nm. It is equivalent to Alexa Fluor™ 680 and Cy5.5 in size, brightness and application. It can be used with the following analysis platforms: Flow Cytometry, In Vivo and Fluorescence Microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 690 nm
- Emission maximum
- 710 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Relative brightness
- 2 of 5
- Quantum yield
- 0.405
PromoFluor 680 has an excitation maximum at 690 nm and an emission maximum at 710 nm, a Stokes shift of 20 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is PromoFluor 680?
- PromoFluor 680 is a fluorophore with an excitation peak at 690 nm and an emission peak at 709 nm. It is equivalent to Alexa Fluor™ 680 and Cy5.5 in size, brightness and application. It can be used with the following analysis platforms: Flow Cytometry, In Vivo and Fluorescence Microscopy.
- What laser excites PromoFluor 680?
- PromoFluor 680 is optimally excited by the 628-640 laser line. Its excitation maximum is 690 nm.
- What filter should I use to detect PromoFluor 680?
- A 695/40 filter captures the peak of the PromoFluor 680 emission near 710 nm with minimal spillover.
- How bright is PromoFluor 680?
- PromoFluor 680 has a relative brightness of 2 out of 5 (quantum yield 0.405).