PE-Fire 780
PE-Fire™ 780 (Ex-Max 565 nm/Em-Max 775 nm)
PE-Fire™ 810 from Biolegend is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Fire™ 810. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Fire™ 810. This tandem has an excitation peak at 565 nm and an emission peak at 803 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 565 nm
- Emission maximum
- 803 nm
- Optimal laser line
- 488-561
- Optimal filter
- 780/60
PE-Fire 780 has an excitation maximum at 565 nm and an emission maximum at 803 nm, a Stokes shift of 238 nm. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is PE-Fire 780?
- PE-Fire™ 810 from Biolegend is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Fire™ 810. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Fire™ 810. This tandem has an excitation peak at 565 nm and an emission peak at 803 nm.
- What laser excites PE-Fire 780?
- PE-Fire 780 is optimally excited by the 488-561 laser line. Its excitation maximum is 565 nm.
- What filter should I use to detect PE-Fire 780?
- A 780/60 filter captures the peak of the PE-Fire 780 emission near 803 nm with minimal spillover.