DyLight 550
DyLight™ 550 (Ex-Max 562 nm/Em-Max 576 nm)
DyLight™ 550 has an excitation peak at 562 nm and an emission peak at 576 nm and is spectrally similar to Alexa Fluor™ 546, Alexa Fluor™ 555, Cy3 and TRITC. DyLight™ 550 is most commonly used in flow cytometery and fluorescence microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 562 nm
- Emission maximum
- 576 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
- Relative brightness
- 2 of 5
- Extinction coefficient
- 150000
- Commercial antibodies
- 20.4k from 14 suppliers
DyLight 550 has an excitation maximum at 562 nm and an emission maximum at 576 nm, a Stokes shift of 14 nm. Its extinction coefficient is 150000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is DyLight 550?
- DyLight™ 550 has an excitation peak at 562 nm and an emission peak at 576 nm and is spectrally similar to Alexa Fluor™ 546, Alexa Fluor™ 555, Cy3 and TRITC. DyLight™ 550 is most commonly used in flow cytometery and fluorescence microscopy applications.
- What laser excites DyLight 550?
- DyLight 550 is optimally excited by the 561 laser line. Its excitation maximum is 562 nm.
- What filter should I use to detect DyLight 550?
- A 585/42 filter captures the peak of the DyLight 550 emission near 576 nm with minimal spillover.
- How bright is DyLight 550?
- DyLight 550 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to DyLight 550?
- FluoroFinder tracks 20416 commercially available DyLight 550 conjugates, searchable by target, clone, host and application.