Live-or-Dye NucFix Red
Live-or-Dye™ NucFix Red (Ex-Max 520 nm/Em-Max 593 nm)
Biotium' Live-or-Dye™ NucFix Red is a fluorogenic, cell-impermeant nucleic acid stain with an excitation peak at 520 nm and an emission peak at 593 nm. Although it is a nuclear stain, it also covalently crosslinks inside the cell for retention with subsequent fixation and permeabilization.
Excitation & emission spectra
Key facts
- Excitation maximum
- 520 nm
- Emission maximum
- 593 nm
- Optimal laser line
- 488/532
- Optimal filter
- 585/42
- Commercial antibodies
- 2 from 1 supplier
Live-or-Dye NucFix Red has an excitation maximum at 520 nm and an emission maximum at 593 nm, a Stokes shift of 73 nm. It is optimally excited by the 488/532 laser line.
Frequently asked questions
- What is Live-or-Dye NucFix Red?
- Biotium' Live-or-Dye™ NucFix Red is a fluorogenic, cell-impermeant nucleic acid stain with an excitation peak at 520 nm and an emission peak at 593 nm. Although it is a nuclear stain, it also covalently crosslinks inside the cell for retention with subsequent fixation and permeabilization.
- What laser excites Live-or-Dye NucFix Red?
- Live-or-Dye NucFix Red is optimally excited by the 488/532 laser line. Its excitation maximum is 520 nm.
- What filter should I use to detect Live-or-Dye NucFix Red?
- A 585/42 filter captures the peak of the Live-or-Dye NucFix Red emission near 593 nm with minimal spillover.
- How many antibodies are available conjugated to Live-or-Dye NucFix Red?
- FluoroFinder tracks 2 commercially available Live-or-Dye NucFix Red conjugates, searchable by target, clone, host and application.