Live-or-Dye 405/545
Live-or-Dye™ 405/545 (Ex-Max 413 nm/Em-Max 547 nm)
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 405/545 has an excitation peak at 405 nm and an emission peak at 545 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 413 nm
- Emission maximum
- 547 nm
- Optimal laser line
- 405
- Optimal filter
- 525/50
- Commercial antibodies
- 2 from 1 supplier
Live-or-Dye 405/545 has an excitation maximum at 413 nm and an emission maximum at 547 nm, a Stokes shift of 134 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Live-or-Dye 405/545?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 405/545 has an excitation peak at 405 nm and an emission peak at 545 nm.
- What laser excites Live-or-Dye 405/545?
- Live-or-Dye 405/545 is optimally excited by the 405 laser line. Its excitation maximum is 413 nm.
- What filter should I use to detect Live-or-Dye 405/545?
- A 525/50 filter captures the peak of the Live-or-Dye 405/545 emission near 547 nm with minimal spillover.
- How many antibodies are available conjugated to Live-or-Dye 405/545?
- FluoroFinder tracks 2 commercially available Live-or-Dye 405/545 conjugates, searchable by target, clone, host and application.