Live-or-Dye 750/777
Live-or-Dye™ 750/777 (Ex-Max 755 nm/Em-Max 779 nm)
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 750/777 has an excitation peak at 750 nm and an emission peak at 777 nm. In flow cytometry, the 633nm laser is typically used for excitation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 755 nm
- Emission maximum
- 779 nm
- Optimal laser line
- 633/750
- Optimal filter
- 780/60
- Commercial antibodies
- 4 from 2 suppliers
Live-or-Dye 750/777 has an excitation maximum at 755 nm and an emission maximum at 779 nm, a Stokes shift of 24 nm. It is optimally excited by the 633/750 laser line.
Frequently asked questions
- What is Live-or-Dye 750/777?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 750/777 has an excitation peak at 750 nm and an emission peak at 777 nm. In flow cytometry, the 633nm laser is typically used for excitation.
- What laser excites Live-or-Dye 750/777?
- Live-or-Dye 750/777 is optimally excited by the 633/750 laser line. Its excitation maximum is 755 nm.
- What filter should I use to detect Live-or-Dye 750/777?
- A 780/60 filter captures the peak of the Live-or-Dye 750/777 emission near 779 nm with minimal spillover.
- How many antibodies are available conjugated to Live-or-Dye 750/777?
- FluoroFinder tracks 4 commercially available Live-or-Dye 750/777 conjugates, searchable by target, clone, host and application.