LysoView 633
LysoView™ 633 (Ex-Max 634 nm/Em-Max 659 nm)
Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 633 has an excitation peak at 633 nm and an emission peak at 659 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 634 nm
- Emission maximum
- 659 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Commercial antibodies
- 2 from 1 supplier
LysoView 633 has an excitation maximum at 634 nm and an emission maximum at 659 nm, a Stokes shift of 25 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is LysoView 633?
- Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 633 has an excitation peak at 633 nm and an emission peak at 659 nm.
- What laser excites LysoView 633?
- LysoView 633 is optimally excited by the 628-640 laser line. Its excitation maximum is 634 nm.
- What filter should I use to detect LysoView 633?
- A 660/20 filter captures the peak of the LysoView 633 emission near 659 nm with minimal spillover.
- How many antibodies are available conjugated to LysoView 633?
- FluoroFinder tracks 2 commercially available LysoView 633 conjugates, searchable by target, clone, host and application.