NucView 530
NucView 530 (Ex-Max 528 nm/Em-Max 563 nm)
NucView 530 Caspase- 3/7 substrate is a cell-permeant substrate for detecting apoptosis in live cells. It has an excitation peak at 528 nm and an emission peak at 563 nm. This method is often paired with an Annexin V conjugate and impermeant nucleic acid stains. It can be used with the following analysis platforms: Fluorescence Microscopy, Fluorescence Microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 528 nm
- Emission maximum
- 563 nm
- Optimal laser line
- 532
- Optimal filter
- 585/42
NucView 530 has an excitation maximum at 528 nm and an emission maximum at 563 nm, a Stokes shift of 35 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is NucView 530?
- NucView 530 Caspase- 3/7 substrate is a cell-permeant substrate for detecting apoptosis in live cells. It has an excitation peak at 528 nm and an emission peak at 563 nm. This method is often paired with an Annexin V conjugate and impermeant nucleic acid stains. It can be used with the following analysis platforms: Fluorescence Microscopy, Fluorescence Microscopy.
- What laser excites NucView 530?
- NucView 530 is optimally excited by the 532 laser line. Its excitation maximum is 528 nm.
- What filter should I use to detect NucView 530?
- A 585/42 filter captures the peak of the NucView 530 emission near 563 nm with minimal spillover.