NucView 488
NucView 488 (Ex-Max 500 nm/Em-Max 530 nm)
NucView 488 Caspase- 3/7 substrate is a cell-permeant substrate for detecting apoptosis in live cells. It has an excitation peak at 500 nm and an emission peak at 530 nm. This method is often paired with an Annexin V conjugate and impermeant nucleic acid stains. It can be used with the following analysis platforms: Fluorescence Microscopy, Fluorescence Microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 500 nm
- Emission maximum
- 530 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
NucView 488 has an excitation maximum at 500 nm and an emission maximum at 530 nm, a Stokes shift of 30 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is NucView 488?
- NucView 488 Caspase- 3/7 substrate is a cell-permeant substrate for detecting apoptosis in live cells. It has an excitation peak at 500 nm and an emission peak at 530 nm. This method is often paired with an Annexin V conjugate and impermeant nucleic acid stains. It can be used with the following analysis platforms: Fluorescence Microscopy, Fluorescence Microscopy.
- What laser excites NucView 488?
- NucView 488 is optimally excited by the 488 laser line. Its excitation maximum is 500 nm.
- What filter should I use to detect NucView 488?
- A 525/50 filter captures the peak of the NucView 488 emission near 530 nm with minimal spillover.