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CF770

CF®770 (Ex-Max 770 nm/Em-Max 797 nm)

CF®770 from Biotium is a fluorophore with an excitation peak at 770 nm and an emission peak at 797 nm. It is spectrally similar to DyeLight 800 and IRDye® 800CW.

Excitation & emission spectra

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Key facts

Excitation maximum
770 nm
Emission maximum
797 nm
Optimal laser line
730-750
Optimal filter
780/60
Relative brightness
4 of 5
Extinction coefficient
220000
Molecular weight
3138
Commercial antibodies
58 from 1 supplier

CF770 has an excitation maximum at 770 nm and an emission maximum at 797 nm, a Stokes shift of 27 nm. Its extinction coefficient is 220000 M⁻¹cm⁻¹. It is optimally excited by the 730-750 laser line.

Frequently asked questions

What is CF770?
CF®770 from Biotium is a fluorophore with an excitation peak at 770 nm and an emission peak at 797 nm. It is spectrally similar to DyeLight 800 and IRDye® 800CW.
What laser excites CF770?
CF770 is optimally excited by the 730-750 laser line. Its excitation maximum is 770 nm.
What filter should I use to detect CF770?
A 780/60 filter captures the peak of the CF770 emission near 797 nm with minimal spillover.
How bright is CF770?
CF770 has a relative brightness of 4 out of 5.
How many antibodies are available conjugated to CF770?
FluoroFinder tracks 58 commercially available CF770 conjugates, searchable by target, clone, host and application.