CF647
CF®647 (Ex-Max 650 nm/Em-Max 665 nm)
CF®647 from Biotium is a fluorophore with an excitation peak at 642 nm and an emission peak at 662 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 647, DyLight™ 649 and Cy5.
Excitation & emission spectra
Key facts
- Excitation maximum
- 650 nm
- Emission maximum
- 665 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 4 of 5
- Extinction coefficient
- 240000
- Molecular weight
- 1058
- Commercial antibodies
- 22.1k from 2 suppliers
CF647 has an excitation maximum at 650 nm and an emission maximum at 665 nm, a Stokes shift of 15 nm. Its extinction coefficient is 240000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is CF647?
- CF®647 from Biotium is a fluorophore with an excitation peak at 642 nm and an emission peak at 662 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 647, DyLight™ 649 and Cy5.
- What laser excites CF647?
- CF647 is optimally excited by the 628-640 laser line. Its excitation maximum is 650 nm.
- What filter should I use to detect CF647?
- A 660/20 filter captures the peak of the CF647 emission near 665 nm with minimal spillover.
- How bright is CF647?
- CF647 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to CF647?
- FluoroFinder tracks 22078 commercially available CF647 conjugates, searchable by target, clone, host and application.