Antibody Search
OMIPs
eLearning 
Dye Directory
CF633
CF®633 (Ex-Max 630 nm/Em-Max 650 nm)
CF®633 from Biotium is a fluorophore with an excitation peak at 630 nm and an emission peak at 650 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 633, DyLight™ 633 and Cy5. In microscopy, CF®633 has been validated in TIRF imaging.
Excitation & emission spectra
Key facts
- Excitation maximum
- 630 nm
- Emission maximum
- 650 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 3 of 5
- Extinction coefficient
- 100000
- Molecular weight
- 820
- Commercial antibodies
- 8 from 1 supplier
CF633 has an excitation maximum at 630 nm and an emission maximum at 650 nm, a Stokes shift of 20 nm. Its extinction coefficient is 100000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is CF633?
- CF®633 from Biotium is a fluorophore with an excitation peak at 630 nm and an emission peak at 650 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 633, DyLight™ 633 and Cy5. In microscopy, CF®633 has been validated in TIRF imaging.
- What laser excites CF633?
- CF633 is optimally excited by the 628-640 laser line. Its excitation maximum is 630 nm.
- What filter should I use to detect CF633?
- A 660/20 filter captures the peak of the CF633 emission near 650 nm with minimal spillover.
- How bright is CF633?
- CF633 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to CF633?
- FluoroFinder tracks 8 commercially available CF633 conjugates, searchable by target, clone, host and application.