CF594ST
CF®594ST (Ex-Max 593 nm/Em-Max 614 nm)
CF®594ST from Biotium is a fluorophore with an excitation peak at 593 nm and an emission peak at 614 nm. It can be useful in both fluorescence microscopy and flow cytometry but was specifically designed for use in super-resolution imaging by STORM. It is spectrally similar to Alexa Fluor™ 594, DyLight™ 594 and Texas Red.
Excitation & emission spectra
Key facts
- Excitation maximum
- 593 nm
- Emission maximum
- 614 nm
- Optimal laser line
- 594
- Optimal filter
- 610/20
- Relative brightness
- 4 of 5
- Extinction coefficient
- 115000
- Molecular weight
- 868
CF594ST has an excitation maximum at 593 nm and an emission maximum at 614 nm, a Stokes shift of 21 nm. Its extinction coefficient is 115000 M⁻¹cm⁻¹. It is optimally excited by the 594 laser line.
Frequently asked questions
- What is CF594ST?
- CF®594ST from Biotium is a fluorophore with an excitation peak at 593 nm and an emission peak at 614 nm. It can be useful in both fluorescence microscopy and flow cytometry but was specifically designed for use in super-resolution imaging by STORM. It is spectrally similar to Alexa Fluor™ 594, DyLight™ 594 and Texas Red.
- What laser excites CF594ST?
- CF594ST is optimally excited by the 594 laser line. Its excitation maximum is 593 nm.
- What filter should I use to detect CF594ST?
- A 610/20 filter captures the peak of the CF594ST emission near 614 nm with minimal spillover.
- How bright is CF594ST?
- CF594ST has a relative brightness of 4 out of 5.