CF535ST
CF®535ST (Ex-Max 535 nm/Em-Max 568 nm)
CF®535ST from Biotium is a fluorophore with an excitation peak at 535 nm and an emission peak at 568 nm. Due to its unique spectral characteristics, it may be useful in spectral flow cytometry but was designed for super-resolution imaging by STORM.
Excitation & emission spectra
Key facts
- Excitation maximum
- 535 nm
- Emission maximum
- 568 nm
- Optimal laser line
- 532
- Optimal filter
- 530/43
- Relative brightness
- 4 of 5
- Extinction coefficient
- 95000
- Molecular weight
- 728
CF535ST has an excitation maximum at 535 nm and an emission maximum at 568 nm, a Stokes shift of 33 nm. Its extinction coefficient is 95000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is CF535ST?
- CF®535ST from Biotium is a fluorophore with an excitation peak at 535 nm and an emission peak at 568 nm. Due to its unique spectral characteristics, it may be useful in spectral flow cytometry but was designed for super-resolution imaging by STORM.
- What laser excites CF535ST?
- CF535ST is optimally excited by the 532 laser line. Its excitation maximum is 535 nm.
- What filter should I use to detect CF535ST?
- A 530/43 filter captures the peak of the CF535ST emission near 568 nm with minimal spillover.
- How bright is CF535ST?
- CF535ST has a relative brightness of 4 out of 5.