CF514
CF®514 (Ex-Max 516 nm/Em-Max 548 nm)
CF®514 from Biotium is a fluorophore with an excitation peak at 516 nm and an emission peak at 548 nm. It is spectrally similar to Alexa Fluor™ 514.
Excitation & emission spectra
Key facts
- Excitation maximum
- 516 nm
- Emission maximum
- 548 nm
- Optimal laser line
- 488
- Optimal filter
- 530/43
- Relative brightness
- 1 of 5
- Extinction coefficient
- 105000
- Molecular weight
- 1216
- Commercial antibodies
- 22 from 1 supplier
CF514 has an excitation maximum at 516 nm and an emission maximum at 548 nm, a Stokes shift of 32 nm. Its extinction coefficient is 105000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is CF514?
- CF®514 from Biotium is a fluorophore with an excitation peak at 516 nm and an emission peak at 548 nm. It is spectrally similar to Alexa Fluor™ 514.
- What laser excites CF514?
- CF514 is optimally excited by the 488 laser line. Its excitation maximum is 516 nm.
- What filter should I use to detect CF514?
- A 530/43 filter captures the peak of the CF514 emission near 548 nm with minimal spillover.
- How bright is CF514?
- CF514 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to CF514?
- FluoroFinder tracks 22 commercially available CF514 conjugates, searchable by target, clone, host and application.