CF450
CF®450 (Ex-Max 450 nm/Em-Max 538 nm)
CF®450 from Biotium is a fluorophore with an excitation peak at 450 nm and an emission peak at 538 nm. Due to its unique spectral characteristics, it can be used in spectral flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 450 nm
- Emission maximum
- 538 nm
- Optimal laser line
- 405
- Optimal filter
- 530/43
- Relative brightness
- 2 of 5
- Extinction coefficient
- 40000
- Molecular weight
- 689
CF450 has an excitation maximum at 450 nm and an emission maximum at 538 nm, a Stokes shift of 88 nm. Its extinction coefficient is 40000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is CF450?
- CF®450 from Biotium is a fluorophore with an excitation peak at 450 nm and an emission peak at 538 nm. Due to its unique spectral characteristics, it can be used in spectral flow cytometry.
- What laser excites CF450?
- CF450 is optimally excited by the 405 laser line. Its excitation maximum is 450 nm.
- What filter should I use to detect CF450?
- A 530/43 filter captures the peak of the CF450 emission near 538 nm with minimal spillover.
- How bright is CF450?
- CF450 has a relative brightness of 2 out of 5.