CF440
CF®440 (Ex-Max 440 nm/Em-Max 515 nm)
CF®440 from Biotium is a fluorophore with an excitation peak at 440 nm and an emission peak at 515 nm. It is spectrally similar to CF®440 and Alexa Fluor™ 430. Due to its unique spectral characteristics, it can be used in spectral flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 440 nm
- Emission maximum
- 515 nm
- Optimal laser line
- 405
- Optimal filter
- 515/30
- Relative brightness
- 1 of 5
- Extinction coefficient
- 40000
- Molecular weight
- 716
CF440 has an excitation maximum at 440 nm and an emission maximum at 515 nm, a Stokes shift of 75 nm. Its extinction coefficient is 40000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is CF440?
- CF®440 from Biotium is a fluorophore with an excitation peak at 440 nm and an emission peak at 515 nm. It is spectrally similar to CF®440 and Alexa Fluor™ 430. Due to its unique spectral characteristics, it can be used in spectral flow cytometry.
- What laser excites CF440?
- CF440 is optimally excited by the 405 laser line. Its excitation maximum is 440 nm.
- What filter should I use to detect CF440?
- A 515/30 filter captures the peak of the CF440 emission near 515 nm with minimal spillover.
- How bright is CF440?
- CF440 has a relative brightness of 1 out of 5.