CF350
CF®350 (Ex-Max 347 nm/Em-Max 448 nm)
CF®350 from Biotium is a fluorophore with an excitation peak at 347 nm and an emission peak at 448 nm. It is spectrally similar to Coumarin, Alexa Fluor™ 350 and DyLight™ 350 and can be use in fluorescence microscopy and flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 347 nm
- Emission maximum
- 448 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
- Relative brightness
- 1 of 5
- Extinction coefficient
- 18000
- Molecular weight
- 496
- Commercial antibodies
- 2 from 1 supplier
CF350 has an excitation maximum at 347 nm and an emission maximum at 448 nm, a Stokes shift of 101 nm. Its extinction coefficient is 18000 M⁻¹cm⁻¹. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is CF350?
- CF®350 from Biotium is a fluorophore with an excitation peak at 347 nm and an emission peak at 448 nm. It is spectrally similar to Coumarin, Alexa Fluor™ 350 and DyLight™ 350 and can be use in fluorescence microscopy and flow cytometry.
- What laser excites CF350?
- CF350 is optimally excited by the 355 laser line. Its excitation maximum is 347 nm.
- What filter should I use to detect CF350?
- A 450/50 filter captures the peak of the CF350 emission near 448 nm with minimal spillover.
- How bright is CF350?
- CF350 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to CF350?
- FluoroFinder tracks 2 commercially available CF350 conjugates, searchable by target, clone, host and application.