What laser excites ATTO 532?

ATTO 532 is optimally excited by the 532 laser line. Its excitation maximum is 532 nm.

What filter should I use to detect ATTO 532?

A 530/43 filter captures the peak of the ATTO 532 emission near 553 nm with minimal spillover.

How bright is ATTO 532?

ATTO 532 has a relative brightness of 1 out of 5 (quantum yield 0.9).

How many antibodies are available conjugated to ATTO 532?

FluoroFinder tracks 21 commercially available ATTO 532 conjugates, searchable by target, clone, host and application.

ATTO 532

ATTO 532 (Ex-Max 532 nm/Em-Max 553 nm)

ATTO 532 from ATTO-TEC has an excitation peak at 532 nm and an emission peak at 553 nm. ATTO 532 is a fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photo-stability, and excellent water solubility. Thus ATTO 532 is highly suitable for single-molecule detection applications and high-resolution microscopy such as SIM, STED etc.

Excitation & emission spectra

Key facts

Excitation maximum: 532 nm
Emission maximum: 553 nm
Optimal laser line: 532
Optimal filter: 530/43
Relative brightness: 1 of 5
Extinction coefficient: 115000
Quantum yield: 0.9
Fluorescence lifetime: 3.8 ns
Molecular weight: 966
Commercial antibodies: 21 from 3 suppliers

ATTO 532 has an excitation maximum at 532 nm and an emission maximum at 553 nm, a Stokes shift of 21 nm. Its extinction coefficient is 115000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.

Frequently asked questions

What is ATTO 532?: ATTO 532 from ATTO-TEC has an excitation peak at 532 nm and an emission peak at 553 nm. ATTO 532 is a fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photo-stability, and excellent water solubility. Thus ATTO 532 is highly suitable for single-molecule detection applications and high-resolution microscopy such as SIM, STED etc.
What laser excites ATTO 532?: ATTO 532 is optimally excited by the 532 laser line. Its excitation maximum is 532 nm.
What filter should I use to detect ATTO 532?: A 530/43 filter captures the peak of the ATTO 532 emission near 553 nm with minimal spillover.
How bright is ATTO 532?: ATTO 532 has a relative brightness of 1 out of 5 (quantum yield 0.9).
How many antibodies are available conjugated to ATTO 532?: FluoroFinder tracks 21 commercially available ATTO 532 conjugates, searchable by target, clone, host and application.