ATTO 425
ATTO 425 (Ex-Max 436 nm/Em-Max 484 nm)
ATTO 425 from ATTO-Tec Gmbh has an excitation peak at 436 nm and an emission peak at 484 nm. ATTO 425 is a novel fluorescent label with a coumarin structure. Characteristic features of the label are high fluorescence quantum yield, large Stokes-shift, high photo-stability, and low molecular weight. ATTO 425 is moderately hydrophilic.
Excitation & emission spectra
Key facts
- Excitation maximum
- 439 nm
- Emission maximum
- 485 nm
- Optimal laser line
- 405, 445
- Optimal filter
- 450/50
- Relative brightness
- 1 of 5
- Extinction coefficient
- 45000
- Quantum yield
- 0.9
- Fluorescence lifetime
- 3.6 ns
- Molecular weight
- 427
- Commercial antibodies
- 23 from 5 suppliers
ATTO 425 has an excitation maximum at 439 nm and an emission maximum at 485 nm, a Stokes shift of 46 nm. Its extinction coefficient is 45000 M⁻¹cm⁻¹. It is optimally excited by the 405, 445 laser line.
Frequently asked questions
- What is ATTO 425?
- ATTO 425 from ATTO-Tec Gmbh has an excitation peak at 436 nm and an emission peak at 484 nm. ATTO 425 is a novel fluorescent label with a coumarin structure. Characteristic features of the label are high fluorescence quantum yield, large Stokes-shift, high photo-stability, and low molecular weight. ATTO 425 is moderately hydrophilic.
- What laser excites ATTO 425?
- ATTO 425 is optimally excited by the 405, 445 laser line. Its excitation maximum is 439 nm.
- What filter should I use to detect ATTO 425?
- A 450/50 filter captures the peak of the ATTO 425 emission near 485 nm with minimal spillover.
- How bright is ATTO 425?
- ATTO 425 has a relative brightness of 1 out of 5 (quantum yield 0.9).
- How many antibodies are available conjugated to ATTO 425?
- FluoroFinder tracks 23 commercially available ATTO 425 conjugates, searchable by target, clone, host and application.