Brilliant Violet 785
Brilliant™ Violet 785 (Ex-Max 405 nm/Em-Max 785 nm)
Brilliant™ Violet 785 (BV785) is a far-red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 780/60 bandpass filter. BV785 has an excitation peak at 405 nm and an emission peak at 785 nm. BV785 exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large Stokes shift, however BV785 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
Excitation & emission spectra
Key facts
- Excitation maximum
- 405 nm
- Emission maximum
- 785 nm
- Optimal laser line
- 405
- Optimal filter
- 780/60
- Relative brightness
- 3 of 5
- Extinction coefficient
- 2500000
- Quantum yield
- 0.04
- Molecular weight
- 60000
- Commercial antibodies
- 753 from 3 suppliers
Brilliant Violet 785 has an excitation maximum at 405 nm and an emission maximum at 785 nm, a Stokes shift of 380 nm. Its extinction coefficient is 2500000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Brilliant Violet 785?
- Brilliant™ Violet 785 (BV785) is a far-red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 780/60 bandpass filter. BV785 has an excitation peak at 405 nm and an emission peak at 785 nm. BV785 exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large Stokes shift, however BV785 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
- What laser excites Brilliant Violet 785?
- Brilliant Violet 785 is optimally excited by the 405 laser line. Its excitation maximum is 405 nm.
- What filter should I use to detect Brilliant Violet 785?
- A 780/60 filter captures the peak of the Brilliant Violet 785 emission near 785 nm with minimal spillover.
- How bright is Brilliant Violet 785?
- Brilliant Violet 785 has a relative brightness of 3 out of 5 (quantum yield 0.04).
- How many antibodies are available conjugated to Brilliant Violet 785?
- FluoroFinder tracks 753 commercially available Brilliant Violet 785 conjugates, searchable by target, clone, host and application.