Brilliant Violet 650
Brilliant™ Violet 650 (Ex-Max 405 nm/Em-Max 645 nm)
Brilliant™ Violet 650 (BV650) is a red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 660/20 bandpass filter. BV650 has an excitation peak at 405 nm and an emission peak at 645 nm. Due to its unique Stokes shift, not many dyes have identical excitation and emission spectrums, but the nearest alternatives include SuperBright 645, Qdot® 655 and eFluor™ 650NC (ThermoFisher). BV650 exhibits a medium level of brightness and is most often used in flow cytometry, specifically, it is an alternative to nanocrystals used for intracellular flow. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
Excitation & emission spectra
Key facts
- Excitation maximum
- 405 nm
- Emission maximum
- 645 nm
- Optimal laser line
- 405
- Optimal filter
- 660/20
- Relative brightness
- 3 of 5
- Extinction coefficient
- 2500000
- Quantum yield
- 0.17
- Molecular weight
- 63000
- Commercial antibodies
- 1.7k from 5 suppliers
Brilliant Violet 650 has an excitation maximum at 405 nm and an emission maximum at 645 nm, a Stokes shift of 240 nm. Its extinction coefficient is 2500000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Brilliant Violet 650?
- Brilliant™ Violet 650 (BV650) is a red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 660/20 bandpass filter. BV650 has an excitation peak at 405 nm and an emission peak at 645 nm. Due to its unique Stokes shift, not many dyes have identical excitation and emission spectrums, but the nearest alternatives include SuperBright 645, Qdot® 655 and eFluor™ 650NC (ThermoFisher). BV650 exhibits a medium level of brightness and is most often used in flow cytometry, specifically, it is an alternative to nanocrystals used for intracellular flow. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
- What laser excites Brilliant Violet 650?
- Brilliant Violet 650 is optimally excited by the 405 laser line. Its excitation maximum is 405 nm.
- What filter should I use to detect Brilliant Violet 650?
- A 660/20 filter captures the peak of the Brilliant Violet 650 emission near 645 nm with minimal spillover.
- How bright is Brilliant Violet 650?
- Brilliant Violet 650 has a relative brightness of 3 out of 5 (quantum yield 0.17).
- How many antibodies are available conjugated to Brilliant Violet 650?
- FluoroFinder tracks 1680 commercially available Brilliant Violet 650 conjugates, searchable by target, clone, host and application.