Brilliant Violet 605
Brilliant™ Violet 605 (Ex-Max 407 nm/Em-Max 602 nm)
Brilliant™ Violet 605 (BV605) is an orange/red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 610/20 bandpass filter. BV605 has an excitation peak at 405 nm and an and emission peak at 603 nm. BV605 is most commonly used in flow cytometry and is spectrally similar to SuperBright 600 (Thermo Fisher), SuperNova v605 (Beckman Coulter) and StarBright Violet 610 (Bio-Rad). BV605 is considered to be relatively bright and should be used on antigens of moderate to low abudance. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
Excitation & emission spectra
Key facts
- Excitation maximum
- 405 nm
- Emission maximum
- 602 nm
- Optimal laser line
- 405
- Optimal filter
- 610/20
- Relative brightness
- 2 of 5
- Extinction coefficient
- 2400000
- Quantum yield
- 0.29
- Molecular weight
- 60000
- Commercial antibodies
- 2.1k from 5 suppliers
Brilliant Violet 605 has an excitation maximum at 405 nm and an emission maximum at 602 nm, a Stokes shift of 197 nm. Its extinction coefficient is 2400000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Brilliant Violet 605?
- Brilliant™ Violet 605 (BV605) is an orange/red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 610/20 bandpass filter. BV605 has an excitation peak at 405 nm and an and emission peak at 603 nm. BV605 is most commonly used in flow cytometry and is spectrally similar to SuperBright 600 (Thermo Fisher), SuperNova v605 (Beckman Coulter) and StarBright Violet 610 (Bio-Rad). BV605 is considered to be relatively bright and should be used on antigens of moderate to low abudance. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
- What laser excites Brilliant Violet 605?
- Brilliant Violet 605 is optimally excited by the 405 laser line. Its excitation maximum is 405 nm.
- What filter should I use to detect Brilliant Violet 605?
- A 610/20 filter captures the peak of the Brilliant Violet 605 emission near 602 nm with minimal spillover.
- How bright is Brilliant Violet 605?
- Brilliant Violet 605 has a relative brightness of 2 out of 5 (quantum yield 0.29).
- How many antibodies are available conjugated to Brilliant Violet 605?
- FluoroFinder tracks 2069 commercially available Brilliant Violet 605 conjugates, searchable by target, clone, host and application.