Brilliant Violet 570
Brilliant™ Violet 570 (Ex-Max 405 nm/Em-Max 570 nm)
Brilliant™ Violet 570 (BV570) is a yellow-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 585/42 bandpass filter. BV570 has an excitation peak at 405 nm and an emission peak at 570 nm, and is spectrally similar to StarBright 570 (Bio-Rad). This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
Excitation & emission spectra
Key facts
- Excitation maximum
- 405 nm
- Emission maximum
- 570 nm
- Optimal laser line
- 405
- Optimal filter
- 585/42
- Relative brightness
- 2 of 5
- Extinction coefficient
- 2300000
- Quantum yield
- 0.08
- Molecular weight
- 60000
- Commercial antibodies
- 207 from 3 suppliers
Brilliant Violet 570 has an excitation maximum at 405 nm and an emission maximum at 570 nm, a Stokes shift of 165 nm. Its extinction coefficient is 2300000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Brilliant Violet 570?
- Brilliant™ Violet 570 (BV570) is a yellow-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 585/42 bandpass filter. BV570 has an excitation peak at 405 nm and an emission peak at 570 nm, and is spectrally similar to StarBright 570 (Bio-Rad). This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
- What laser excites Brilliant Violet 570?
- Brilliant Violet 570 is optimally excited by the 405 laser line. Its excitation maximum is 405 nm.
- What filter should I use to detect Brilliant Violet 570?
- A 585/42 filter captures the peak of the Brilliant Violet 570 emission near 570 nm with minimal spillover.
- How bright is Brilliant Violet 570?
- Brilliant Violet 570 has a relative brightness of 2 out of 5 (quantum yield 0.08).
- How many antibodies are available conjugated to Brilliant Violet 570?
- FluoroFinder tracks 207 commercially available Brilliant Violet 570 conjugates, searchable by target, clone, host and application.