Brilliant Violet 510
Brilliant™ Violet 510 (Ex-Max 405 nm/Em-Max 510 nm)
Brilliant™ Violet 510 (BV510) is a green-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 510/50 bandpass filter. BV510 has an excitation peak at 405 nm and an emission peak at 510 nm, and is similar to StarBright Violet 515 (Bio-Rad). BV510 is most often used in flow cytometry due to its improved signal-to-noise over alternatives like V500 and AmCyan. This dye is part of the Brilliant™ Violet dye line. BV510 and BV480 are the only dyes in the Brilliant™ Violet dye family that are not tandem fluorophores based off the BV421 polymer core, but they have nearly the same maximum excitation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 405 nm
- Emission maximum
- 510 nm
- Optimal laser line
- 405
- Optimal filter
- 510/50
- Relative brightness
- 2 of 5
- Extinction coefficient
- 577000
- Quantum yield
- 0.44
- Molecular weight
- 77000
- Commercial antibodies
- 1.6k from 5 suppliers
Brilliant Violet 510 has an excitation maximum at 405 nm and an emission maximum at 510 nm, a Stokes shift of 105 nm. Its extinction coefficient is 577000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Brilliant Violet 510?
- Brilliant™ Violet 510 (BV510) is a green-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 510/50 bandpass filter. BV510 has an excitation peak at 405 nm and an emission peak at 510 nm, and is similar to StarBright Violet 515 (Bio-Rad). BV510 is most often used in flow cytometry due to its improved signal-to-noise over alternatives like V500 and AmCyan. This dye is part of the Brilliant™ Violet dye line. BV510 and BV480 are the only dyes in the Brilliant™ Violet dye family that are not tandem fluorophores based off the BV421 polymer core, but they have nearly the same maximum excitation.
- What laser excites Brilliant Violet 510?
- Brilliant Violet 510 is optimally excited by the 405 laser line. Its excitation maximum is 405 nm.
- What filter should I use to detect Brilliant Violet 510?
- A 510/50 filter captures the peak of the Brilliant Violet 510 emission near 510 nm with minimal spillover.
- How bright is Brilliant Violet 510?
- Brilliant Violet 510 has a relative brightness of 2 out of 5 (quantum yield 0.44).
- How many antibodies are available conjugated to Brilliant Violet 510?
- FluoroFinder tracks 1630 commercially available Brilliant Violet 510 conjugates, searchable by target, clone, host and application.