DY-520XL
DY-520XL (Ex-Max 517 nm/Em-Max 663 nm)
DY-520XL from Dyomics is a member of their MegaStokes fluorophore family. These dyes are characterized by very dramatic Stokes Shifts between their excitation and emission peaks. This fluorophore has an excitation peak at 520 nm and an emission peak at 664 nm. Although spectrally they could be applied to flow cytometry applications, they exhibit wide emission ranges that will spillover into neighboring emission channels. They are most commonly used for fluorescence microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 520 nm
- Emission maximum
- 664 nm
- Optimal laser line
- 488
- Optimal filter
- 660/20
- Relative brightness
- 2 of 5
- Commercial antibodies
- 1 from 1 supplier
DY-520XL has an excitation maximum at 520 nm and an emission maximum at 664 nm, a Stokes shift of 144 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is DY-520XL?
- DY-520XL from Dyomics is a member of their MegaStokes fluorophore family. These dyes are characterized by very dramatic Stokes Shifts between their excitation and emission peaks. This fluorophore has an excitation peak at 520 nm and an emission peak at 664 nm. Although spectrally they could be applied to flow cytometry applications, they exhibit wide emission ranges that will spillover into neighboring emission channels. They are most commonly used for fluorescence microscopy.
- What laser excites DY-520XL?
- DY-520XL is optimally excited by the 488 laser line. Its excitation maximum is 520 nm.
- What filter should I use to detect DY-520XL?
- A 660/20 filter captures the peak of the DY-520XL emission near 664 nm with minimal spillover.
- How bright is DY-520XL?
- DY-520XL has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to DY-520XL?
- FluoroFinder tracks 1 commercially available DY-520XL conjugates, searchable by target, clone, host and application.