Brilliant Violet 421
Brilliant™ Violet 421 (Ex-Max 405 nm/Em-Max 421 nm)
Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad.
Excitation & emission spectra
Key facts
- Excitation maximum
- 405 nm
- Emission maximum
- 421 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Relative brightness
- 5 of 5
- Extinction coefficient
- 2500000
- Quantum yield
- 0.65
- Molecular weight
- 70000
- Commercial antibodies
- 3.4k from 8 suppliers
Brilliant Violet 421 has an excitation maximum at 405 nm and an emission maximum at 421 nm, a Stokes shift of 16 nm. Its extinction coefficient is 2500000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Brilliant Violet 421?
- Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad.
- What laser excites Brilliant Violet 421?
- Brilliant Violet 421 is optimally excited by the 405 laser line. Its excitation maximum is 405 nm.
- What filter should I use to detect Brilliant Violet 421?
- A 450/50 filter captures the peak of the Brilliant Violet 421 emission near 421 nm with minimal spillover.
- How bright is Brilliant Violet 421?
- Brilliant Violet 421 has a relative brightness of 5 out of 5 (quantum yield 0.65).
- How many antibodies are available conjugated to Brilliant Violet 421?
- FluoroFinder tracks 3404 commercially available Brilliant Violet 421 conjugates, searchable by target, clone, host and application.