Hoechst (34580)
Hoechst (34580) (Ex-Max 371 nm/Em-Max 440 nm)
Hoechst (34580) is a cell-permeant, fluorogenic nucleic acid stain. It has an excitation peak at 371 nm and an emission peak at 440 nm. It is useful in counter-staining nuclei in both flow cytometry and microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 371 nm
- Emission maximum
- 440 nm
- Optimal laser line
- 355
- Optimal filter
- 450/20
- Commercial antibodies
- 1 from 1 supplier
Hoechst (34580) has an excitation maximum at 371 nm and an emission maximum at 440 nm, a Stokes shift of 69 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Hoechst (34580)?
- Hoechst (34580) is a cell-permeant, fluorogenic nucleic acid stain. It has an excitation peak at 371 nm and an emission peak at 440 nm. It is useful in counter-staining nuclei in both flow cytometry and microscopy.
- What laser excites Hoechst (34580)?
- Hoechst (34580) is optimally excited by the 355 laser line. Its excitation maximum is 371 nm.
- What filter should I use to detect Hoechst (34580)?
- A 450/20 filter captures the peak of the Hoechst (34580) emission near 440 nm with minimal spillover.
- How many antibodies are available conjugated to Hoechst (34580)?
- FluoroFinder tracks 1 commercially available Hoechst (34580) conjugates, searchable by target, clone, host and application.