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FVS575V

FVS575V (Ex-Max 395 nm/Em-Max 568 nm)

FVS575V (Fixable Viability Stain 575V) from BD Biosciences is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 395 nm and an emission peak at 568 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
395 nm
Emission maximum
568 nm
Optimal laser line
405
Optimal filter
585/42
Commercial antibodies
1 from 1 supplier

FVS575V has an excitation maximum at 395 nm and an emission maximum at 568 nm, a Stokes shift of 173 nm. It is optimally excited by the 405 laser line.

Frequently asked questions

What is FVS575V?
FVS575V (Fixable Viability Stain 575V) from BD Biosciences is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 395 nm and an emission peak at 568 nm.
What laser excites FVS575V?
FVS575V is optimally excited by the 405 laser line. Its excitation maximum is 395 nm.
What filter should I use to detect FVS575V?
A 585/42 filter captures the peak of the FVS575V emission near 568 nm with minimal spillover.
How many antibodies are available conjugated to FVS575V?
FluoroFinder tracks 1 commercially available FVS575V conjugates, searchable by target, clone, host and application.